15 Nga Momo o te Human Papillomavirus E6/E7 Gene mRNA
ingoa hua
HWTS-CC005A-15 Nga Momo o te Papillomavirus Tangata E6/E7 Ira ira MRNA Kit Rapu (Fluorescence PCR)
Epidemiology
Ko te mate pukupuku servikal tetahi o nga momo mate pukupuku wahine e tino kitea ana i te ao katoa, a, he tata te papillomavirus o te tangata (HPV), engari he iti noa te wahanga o nga mate HPV ka puta hei mate pukupuku.Ko te HPV morearea nui ka pangia nga pūtau epithelial cervical me te whakaputa e rua oncoproteins, E6 me E7.Ka taea e tenei pūmua te pa ki te maha o nga pūmua pūtau (pērā i te pūmua suppressor tumor pRB me p53), ka whakaroa i te huringa pūtau, ka pa ki te whakahiato DNA me te pumau o te ira, me te whakararu i nga urupare parepare antiviral me te antitumor.
Hongere
| Hongere | Waehanga | I whakamatauria te momoira |
| FAM | Pūrei Tauhohenga HPV 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Ira β-actin tangata | |
| FAM | Pupuri Tauhohenga HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Ira INS tangata |
Tawhā Hangarau
| Rokiroki | Wai: ≤-18 ℃ |
| Papa-ora | 9 marama |
| Momo Tauira | pūtau exfoliated cervical |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kape/mL |
| Nga taputapu e tika ana | Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent Tangohanga Manakohia: Tohutono & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Me tino whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi .Ko te 50μL te rōrahi elution tūtohu.Ki te kore te tauira i tino keri, whakahokia ki te taahiraa 4 mo te kena ano.Na ka whakamatau kia rite ki nga tohutohu mo te whakamahi.
Reagent tangohanga Manakohia: RNAprep Pure Animal Tissue Tapeke RNA Extraction Kit (DP431).Me whakahaere te tangohanga kia rite ki nga tohutohu mo te whakamahi tino (I roto i te taahiraa 5, whawhati te kukū o te otinga mahi DNaseI, ara, tango 20μL o RNase-Free DNaseI (1500U) otinga kararehe ki roto i te ngongo centrifuge RNase-Free hou, tāpirihia te 60μL o te RDD buffer, ka uru ata).Ko 60μL te rōrahi elution tūtohu.Ki te kore te tauira i tino keri, whakahokia ki te taahiraa 5 mo te kena ano.Na ka whakamatau kia rite ki nga tohutohu mo te whakamahi.
