Tangata CYP2C9 me VKORC1 Gene Polymorphism
ingoa hua
HWTS-GE014A-Human CYP2C9 me VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Tiwhikete
CE
Epidemiology
Ko te Warfarin he anticoagulant waha e whakamahia ana i roto i nga mahi haumanu i tenei wa, ko te tikanga mo te aukati me te maimoatanga o nga mate thromboembolic.Heoi, he iti noa te matapihi maimoatanga o te warfarin, he rereke te rereke i waenga i nga iwi me nga tangata takitahi.Kua tohuhia e nga tatauranga ko te rereketanga o te horopeta pumau i roto i nga taangata rereke ka neke atu i te 20 nga wa.Ko te toto tauhohenga kino ka puta ki te 15.2% o nga turoro e tango ana i te warfarin ia tau, ko te 3.5% o enei ka puta he toto mate.Kua whakaatuhia e nga rangahau Pharmacogenomic ko te polymorphism ira o te whaariki whainga VKORC1 me te enzyme metabolic CYP2C9 o te warfarin he take nui e pa ana ki te rereketanga o te horopeta o te warfarin.Ko te Warfarin he aukati motuhake o te huaora K epoxide reductase (VKORC1), na reira ka aukati i te whakahiatotanga tauwehe kawa e uru ana ki te huaora K me te whakarato anticoagulation.He maha nga rangahau kua tohu ko te polymorphism ira o te kaiwhakatairanga VKORC1 te mea tino nui e pa ana ki te iwi me nga rereketanga takitahi i roto i te horopeta e hiahiatia ana o te warfarin.Ko te Warfarin ka whakakorikorihia e te CYP2C9, a ko ona mutants he tino puhoi te paopaotanga o te warfarin.Ko nga tangata takitahi e whakamahi ana i te warfarin he nui ake te mate (e rua ki te toru nga wa teitei ake) o te whakaheke toto i te timatanga o te whakamahinga.
Hongere
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Tawhā Hangarau
| Rokiroki | Wai: ≤-18 ℃ |
| Papa-ora | 12 marama |
| Momo Tauira | Te toto anticoagulated EDTA hou |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Tauwhāiti | Karekau he tauhohenga whakawhiti me etahi atu raupapa tino rite o te ira tangata (ira CYP2C19 tangata, ira RPN2 tangata);te whakarereketanga o CYP2C9*13 me VKORC1 (3730G>A) i waho o te awhe kitenga o tenei kete |
| Nga taputapu e tika ana | Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
Reagent Tangohanga Manakohia: Tonotono & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) me te Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
