Mycobacterium Tuberculosis INH Ātete
Ingoa Hua
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)
Epidemiology
Ko te Isoniazid, he rongoa mo te mate kohi matua i whakaurua mai i te tau 1952, ko tetahi o nga rongoa tino whai hua mo te maimoatanga whakakotahi o te mate kohi kaha me te rongoa kotahi mo te mate kohi huna.
Ko KatG te ira matua e whakawaehere ana i te catalase-peroxidase me te ira ira o te katG ka taea te whakatairanga i te whakahiatotanga o te pakitara pūtau waikawa mycolic, ka aukati te huakita ki te isoniazid.Ko te korero a KatG he pai te hono ki nga huringa i roto i te INH-MIC, me te hekenga 2 o te korero katG ka hua te pikinga 2-nui ake o te MIC.Ko tetahi atu take o te aukati isoniazid i roto i te mycobacterium tuberculosis ka puta i te whakaurunga, te whakakore, te whakarereketanga ranei i te waahi ira InhA o te mycobacterium tuberculosis.
Hongere
| ROX | inhA (-15C>T) pae· |
| CY5 | KatG (315G>C) pae |
| VIC (HEX) | IS6110 |
Tawhā Hangarau
| Rokiroki | ≤-18 ℃ I te pouri |
| Papa-ora | 12 marama |
| Momo Tauira | Te pupuhi |
| CV | ≤5.0% |
| LoD | 1 × 103huakita/mL |
| Tauwhāiti | Ko te tauhohenga kore-ripeka me nga huringa o nga waahi aukati tarukino e wha (511, 516, 526 me 531) o te ira rpoB kei waho o te awhe rapunga o te kete rapu. Nga taputapu e tika ana: Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturu Whakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere QuantStudio®5 Pūnaha PCR Wā-tūturu SLAN-96P Pūnaha PCR Wā-tūturu LightCycler®480 Pūnaha PCR Wā-tūturu LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha BioRad CFX96 Pūnaha PCR Wā-tūturu BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu |
Rere Mahi
