Tangata ROS1 Fusion Gene Mutation

Whakaahuatanga Poto:

Ka whakamahia tenei kete ki te rapunga kounga in vitro o nga momo 14 o nga ira ira whakakotahitanga ROS1 i roto i nga tauira mate pukupuku pūkahukahu kore-iti (Ripanga 1).Ko nga hua whakamatautau mo te tohutoro haumanu anake, kaua e whakamahia hei kaupapa mo te maimoatanga takitahi o nga turoro.


Taipitopito Hua

Tohu Hua

ingoa hua

HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Tiwhikete

CE

Epidemiology

Ko te ROS1 he tyrosine kinase transmembrane o te whanau kaikawe insulin.Kua whakapumautia te ira whakakotahitanga ROS1 hei ira taraiwa mate pukupuku pūkahukahu kore-iti.Hei māngai o te momo iti rāpoi ahurei hou, ko te paheketanga o te ira whakakotahitanga ROS1 i roto i te NSCLC Mo te 1% ki te 2% ROS1 te nuinga ka whakareri i te ira i roto i ona exons 32, 34, 35 me te 36. I muri i te whakakotahitanga ki nga ira penei i te CD74, EZR, SLC34A2, me SDC4, ka kaha tonu te mahi i te rohe ROS1 tyrosine kinase.Ka taea e te kinase ROS1 kua whakakorehia te whakahoahohia nga huarahi tohu o raro penei i te RAS/MAPK/ERK, PI3K/Akt/mTOR, me te JAK3/STAT3, na reira ka uru atu ki te whakatipuranga, te rereketanga me te metastasis o nga pūtau puku, me te mate pukupuku.I roto i te ROS1 fusion irakētanga, CD74-ROS1 kaute mo te 42%, EZR mo te 15%, SLC34A2 mo te 12%, me te SDC4 mo te 7%.Kua whakaatuhia e nga rangahau ko te waahi ATP-binding o te rohe catalytic o ROS1 kinase me te ATP-binding site o ALK kinase he homology ki runga ki te 77%, na te ALK tyrosine kinase iti mole ngota aukati crizotinib me etahi atu ka kitea he painga whakaora. i roto i te maimoatanga o te NSCLC me te whakawhitinga whakawhitinga o ROS1.No reira, ko te kitenga o nga huringa whakakotahitanga ROS1 te kaupapa me te kaupapa hei arahi i te whakamahinga o nga raau taero crizotinib.

Hongere

FAM Tauhohenga Kaipupuri 1, 2, 3 me te 4
VIC(HEX) Tauhohenga Kaipupuri 4

Tawhā Hangarau

Rokiroki

≤-18 ℃

Papa-ora

9 marama

Momo Tauira

Ko te kiko pathological kua mau ki te paraffin, he tauira tapatapahi ranei

CV

<5.0%

Ct

≤38

LoD

Ka taea e tenei kete te kite i nga whakarereketanga whakakotahitanga iti iho i te 20 kape.

Nga taputapu e tika ana:

Whakamahinga Biosystems 7500 Pūnaha PCR Wā-tūturuWhakamahi Biosystems 7500 Pūnaha PCR Wā-tūturu Tere

SLAN ®-96P Pūnaha PCR Wā-tūturu

QuantStudio™ 5 Pūnaha PCR Wā-tūturu

LightCycler®480 Pūnaha PCR Wā-tūturu

LineGene 9600 Plus Pūnaha Tirohanga PCR Wā-tūturu

MA-6000 Waa-Tuturu Ine Ine Hukarere Ngawha

BioRad CFX96 Pūnaha PCR Wā-tūturu

BioRad CFX Opus 96 Pūnaha PCR Wā-tūturu

Rere Mahi

Reagent tangohanga Manakohia: RNeasy FFPE Kit (73504) mai i QIAGEN, Paraffin Embedded Tissue Wāhanga Tapeke RNA Extraction Kit(DP439) mai i Tiangen Biotech(Beijing) Co., Ltd.


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